AI Article Synopsis
- The objective was to develop a High-Performance Liquid Chromatography (HPLC) method to analyze two specific compounds in the bark of Paeonia Suffuticosa.
- The method used a reversed-phase HPLC system with a specific column and mobile phase, operating at a flow rate of 0.8 mL/min and UV detection at 274 nm.
- Results showed strong linearity for both compounds tested and high average recovery rates, indicating the method is effective, simple, and suitable for assessing the quality of the bark.
Article Abstract
Objective: To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.
Method: The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.
Result: The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.
Conclusion: The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.
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