AI Article Synopsis
- Pseudomonas putida NA-1 shows distinct cultivation and enzyme production features compared to S. marcescens IFO 12648 and P. fluorescens TN5.
- The optimal conditions for converting nicotinic acid to 6-hydroxynicotinic acid with Pseudomonas putida NA-1 include a 3% concentration of nicotinic acid, a temperature of 35 degrees Celsius, and a pH of 7.0.
- In a 4-liter fermenter, these conditions resulted in a production yield of 108.39 g/L of 6-hydroxynicotinic acid.
Article Abstract
Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35 degrees C and pH 7.0. Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39 g/L.
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