Five different enterotoxins and one emetic toxin of Bacillus cereus have been characterized. To amplify all of the enterotoxin and emetic-specific sequences of the species in the B. cereus group, a multiplex PCR with 12 primer pairs was established. In developing the assay method, a common terminal sequence at the 3' ends of all primers was chosen and a hot start Taq polymerase was used to overcome primer dimer formation. The assay was successfully applied to analyze the toxigenic potential of 162 food-poisoning and food-related strains. Results showed that there were 10 toxigenic patterns for all the test strains. All of the B. cereus strains carried at least one toxin gene. More than 70% of Bacillus mycoides strains carried no known toxin genes. The toxin profiles and toxin genes of B. mycoides strains were significantly different from B. cereus strains (P < 0.05), although the two species were closely related. The results suggest that many B. mycoides strains might be less prone to cause food poisoning. They also indicate the importance of detecting the toxin genes together with the detection of the species in the B. cereus group.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028x-68.10.2123 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel Anti-Microbial/Anti-Inflammatory Combination Improves Clinical Outcome of Bacillus cereus Endophthalmitis.
Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology, Dean McGee Eye Institute, Oklahoma City, Oklahoma, United States.
Purpose: The purpose of this study was to explore the therapeutic potential of the novel combination of Bacillus bacteriophage lysin (PlyB) and a synthetic TLR2/4 inhibitor (oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine, OxPAPC) in the treatment of experimental Bacillus cereus endophthalmitis.
Methods: C57BL/6J mice were injected with 100 colony forming units (CFUs) Bacillus cereus to induce endophthalmitis. Two hours postinfection, groups of mice were treated with either PlyB, PlyB with OxPAPC, or the groups were left untreated to serve as a control.
Biocomposite Active Whey Protein Films with Thyme Reinforced by Electrospun Polylactic Acid Fiber Mat.
Foods
January 2025
Center of Excellence Polymer Processing, Faculty of Engineering, Dunarea de Jos University of Galați, Domnească Street, No. 111, 800201 Galați, Romania.
Electrospinning is a versatile technique for obtaining nano/micro fibers which are able to significantly change the active properties of composite materials and bring in new dimensions to agri-food applications. Composite bio-based packaging materials obtained from whey proteins, functionalized with thyme essential oil (TEO) and reinforced by electrospun polylactic acid (PLA) fibers, represent a promising solution for developing new active food packaging using environmentally friendly materials. The aim of this study is to obtain and characterize one-side-active composite films covered with a PLA fiber mat: (i) WF/G1, WF/G2, and WF/G3 resulting from electrospinning with one needle at different electrospinning times of 90, 150, and 210 min, respectively, and (ii) WF/G4 obtained with two face-to-face needles after 210 min of electrospinning.
View Article and Find Full Text PDFExploring the intricate studies on low-density polyethylene (LDPE) biodegradation by Bacillus cereus AP-01, isolated from the gut of Styrofoam-fed Tenebrio molitor larvae.
Biodegradation
January 2025
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamilnadu, 608502, India.
Article Synopsis
- The study investigates how well a gut bacteria strain, Bacillus cereus AP-01, can break down low-density polyethylene (LDPE), using experiments over 28 days to measure its effectiveness.
- The researchers employed various methods like FTIR and SEM to analyze changes in LDPE structure and confirmed the bacterial strain through molecular characterization.
- Results showed that the bacteria significantly degraded LDPE, with a 30.3% weight loss and changes in mechanical properties, highlighting its potential as a solution for plastic pollution.
Biofabrication of Silver Nanoparticles Using Extract and Evaluation of Their Antibacterial, Antioxidant, and Cytotoxic Properties.
Life (Basel)
December 2024
Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
The biosynthesis of silver nanoparticles using plant extracts is a promising field of research because of the useful biomedical applications of metal nanoparticles. In this study, the antibacterial and antioxidant properties of silver nanoparticles biosynthesized with the aqueous leaf extract of were defined using a simple, eco-friendly, consistent, and cost-effective method. The leaf extract of (PT) served as a capping and reducing agent to biosynthesize silver nanoparticles.
View Article and Find Full Text PDFEnhancing maize drought and heat tolerance: single vs combined plant growth promoting rhizobacterial inoculation.
Front Plant Sci
December 2024
Unit for Environment Science and Management, North-West University, Potchefstroom, South Africa.
Maize ( L.), a key staple crop in Sub-Saharan Africa, is particularly vulnerable to concurrent drought and heat stress, which threatens crop yield and food security. Plant growth-promoting rhizobacteria (PGPR) have shown potential as biofertilizers to enhance plant resilience under such abiotic stresses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!