AI Article Synopsis
- An enzyme-based glucose biosensor uses a modified xerogel microarray to release nitric oxide (NO) and improve sensitivity to glucose.
- The biosensor features glucose oxidase (GOx) embedded in a protective methyltrimethoxysilane (MTMOS) layer, with polyurethane coatings that prevent enzyme loss and enhance glucose selectivity.
- Micropatterned design facilitates effective NO release while maintaining GOx activity, effectively reducing bacterial adhesion and promoting stability in glucose detection.
Article Abstract
An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported.
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| http://dx.doi.org/10.1016/j.bios.2005.01.010 | DOI Listing |
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