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and Proteins Recognized by Sera of Challenged Pigs.
Front Microbiol
May 2017
CSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, Spain.
The spirochetes and are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS.
View Article and Find Full Text PDFArtificially designed recombinant protein composed of multiple epitopes of foot-and-mouth disease virus as a vaccine candidate.
Microb Cell Fact
February 2017
Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
Article Synopsis
- - Concerns have emerged about the safety of traditional inactivated foot-and-mouth disease (FMD) vaccines, leading to research on a safer alternative using recombinant protein technology.
- - The study developed a chimeric recombinant protein called 5BT, which includes multiple B cell and T cell epitopes to enhance immunogenicity, and was further improved for stability by conjugating it with a membrane protein (BmpB).
- - Immunization of mice with either 5BT or its conjugate B5BT produced strong immune responses, comparable to conventional vaccines, suggesting that this recombinant protein could serve as an effective alternative for FMD vaccination.
Lactobacilli antagonize the growth, motility, and adherence of Brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis.
Appl Environ Microbiol
August 2011
Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom.
Avian intestinal spirochetosis (AIS) results from the colonization of the ceca and colorectum of poultry by pathogenic Brachyspira species. The number of cases of AIS has increased since the 2006 European Union ban on the use of antibiotic growth promoters, which, together with emerging antimicrobial resistance in Brachyspira, has driven renewed interest in alternative intervention strategies. Probiotics have been reported as protecting livestock against infection with common enteric pathogens, and here we investigate which aspects of the biology of Brachyspira they antagonize in order to identify possible interventions against AIS.
View Article and Find Full Text PDFGenetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum.
Antimicrob Agents Chemother
August 2006
Laboratoire de Bactériologie et Epidémiologie Moléculaire, Université de Nice, Centre Hospitalier Universitaire de Nice, France.
Previous studies have reported beta-lactamase-mediated penicillin resistance in Fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. An F. nucleatum subsp.
View Article and Find Full Text PDFSurvival of intestinal spirochaete strains from chickens in the presence of disinfectants and in faeces held at different temperatures.
Avian Pathol
December 2003
School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia, Australia.
This study aimed to evaluate the efficacy of some commonly used disinfectants in inactivating the pathogenic avian intestinal spirochaetes Brachyspira intermedia and Brachyspira pilosicoli, and to examine spirochaete survival in chicken caecal faeces held at 4 degrees C, 25 degrees C or 37 degrees C. Six disinfectants were evaluated at their recommended working concentrations: alkaline salts, quaternary ammonium, iodine as an iodophor, chlorine from a chlorine-release agent, glutaraldehyde and hydrogen peroxide. All but alkaline salts inactivated two different concentrations of both spirochaete species in less than 1 min in the presence of organic matter.
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