Porphyromonas gingivalis is a periodontopathogen that may play a role in cardiovascular diseases. Hemagglutinins may function as adhesins and are required for virulence of several bacterial pathogens. The aim of this study was to determine the role of hemagglutinin B (HagB) in adherence of P. gingivalis to human coronary artery endothelial (HCAE) cells. P. gingivalis strain 381, a P. gingivalis 381 HagB mutant, Escherichia coli JM109 expressing HagB (E. coli-HagB), and E. coli JM109 containing pUC9 (E. coli-pUC9) were tested for their ability to attach to HCAE cells. Inhibition assays were performed to determine the ability of purified recombinant HagB (rHagB) as well as antibodies to HagB, including the polyclonal antibody (PAb) A7985 and the monoclonal antibody (MAb) HL1858, to inhibit the attachment of P. gingivalis to HCAE cells. As expected, when the attachment of P. gingivalis and the HagB mutant were compared, no statistical significance was observed between the two groups (P = 0.331), likely due to the expression of the hagB homolog hagC. However, E. coli-HagB adhered significantly better to HCAE cells than did E. coli-pUC9, the control strain. In a competition assay, the presence of purified rHagB decreased bacterial adhesion of P. gingivalis or E. coli-HagB to HCAE cells. The presence of PAb A7985 or MAb HL1858 also significantly decreased attachment of P. gingivalis and E. coli-HagB to host cells. These results indicate that HagB is involved in the adherence of P. gingivalis to human primary endothelial cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1273858 | PMC |
| http://dx.doi.org/10.1128/IAI.73.11.7267-7273.2005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Developing NIR xanthene-chalcone fluorophores with large Stokes shifts for fluorescence imaging.
Analyst
June 2024
Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, P. R. China.
Article Synopsis
- - A new series of near-infrared xanthene-chalcone fluorophores were developed for fluorescence imaging in live cells, showcasing benefits like low toxicity and large Stokes shifts.
- - The HCA-E fluorophore has a hydroxyl group that allows for the creation of multifunctional fluorescent probes, particularly for detecting glutathione (GSH).
- - The N-HCA-E probe exhibits "turn-on" fluorescence at 709 nm, specifically responding to GSH in lysosomes with a significant Stokes shift and high resistance to interference.
Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation.
PLoS One
June 2023
Department of Biosciences and Bioengineering, Indian Institute of Technology-Guwahati, Guwahati, Assam, India.
Haritaki churna (HC), a single herb ayurvedic formulations is known to be prescribed for various gastro-intestinal disorders in Ayurveda. Haritaki churna aqueous extract (HCAE) has anti-cancer activity against different types of cancer cells with an IC50 in the range of 50-97 μg/ml. Bioavailability of Haritaki Churna is very high in digestive track and treatment of colorectal cancer cells HCT-116, DLD1, HT-29 with HCAE reduces its cellular viability with anti-cancer IC50 70μg/ml.
View Article and Find Full Text PDFCharacterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.
Appl Environ Microbiol
January 2022
Faculty of Engineering and Natural Sciences, Hervanta Campus, Tampere University, Tampere, Finland.
Adaptive laboratory evolution (ALE) is a powerful approach for improving phenotypes of microbial hosts. Evolved strains typically contain numerous mutations that can be revealed by whole-genome sequencing. However, determining the contribution of specific mutations to new phenotypes is typically challenging and laborious.
View Article and Find Full Text PDFModulation of the Host Cell Transcriptome and Epigenome by Fusobacterium nucleatum.
mBio
October 2021
Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
Fusobacterium nucleatum is a ubiquitous opportunistic pathogen with an emerging role as an oncomicrobe in colorectal cancer and other cancer settings. F. nucleatum can adhere to and invade host cells in a manner that varies across F.
View Article and Find Full Text PDFHIV, antiretroviral treatment, hypertension, and stroke in Malawian adults: A case-control study.
Neurology
January 2016
From the Malawi-Liverpool-Wellcome Trust Clinical Research Programme (L.A.B., E.L.C., A.C., R.S.H.) and the Department of Medicine (L.A.B., H.M., S.K., R.S.H., T.J.A.), College of Medicine, University of Malawi, Blantyre; the Brain Infections Group (L.A.B., T.S.), Institute of Infection and Global Health, University of Liverpool (L.A.B., H.C.A.E., T.S.); the Walton Centre NHS Foundation Trust (L.A.B., T.S.), Liverpool; the Department of Clinical Research (E.L.C.), London School of Hygiene and Tropical Medicine; the NHS Borders and Division of Clinical Neuroscience (M.D.C.), University of Edinburgh, UK; the School of Public Health (M.D.C.), University of the Witwatersrand, Johannesburg, South Africa; the Royal Liverpool Hospital (M.H.); the Royal Preston Hospital (H.C.A.E.), Liverpool, UK; the Department of Medicine (A.B.), Division of Neurology, Groote Schuur Hospital, University of Cape Town, South Africa; the Liverpool School of Tropical Medicine (B.F.); the Division of Infection and Immunity (R.S.H.), University College London; and the National Institute for Health Research (T.S.), Health Protection Research Unit in Emerging and Zoonotic Infections, Liverpool, UK.
Objective: To investigate HIV, its treatment, and hypertension as stroke risk factors in Malawian adults.
Methods: We performed a case-control study of 222 adults with acute stroke, confirmed by MRI in 86%, and 503 population controls, frequency-matched for age, sex, and place of residence, using Global Positioning System for random selection. Multivariate logistic regression models were used for case-control comparisons.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!