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Trichomonas vaginalis: identification of a triacylglycerol acylhydrolase. | LitMetric

Trichomonas vaginalis: identification of a triacylglycerol acylhydrolase.

Exp Parasitol

Universidade Católica de Brasília, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, SGAN 916, Brasília DF 70790-160, Brazil.

Published: December 2005

This work describes the identification of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing significant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one amino acid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.

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Source
http://dx.doi.org/10.1016/j.exppara.2005.08.007DOI Listing

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