Progress and changes in embryo technology in farm animals are briefly reviewed in terms of how well embryos can be made and used and what the subject has taught us about the maintenance of pregnancy and reproduction in general. Generalizations are made about the need to not accept dogma, how initially complex techniques always become simplified and thereby more applicable, and the need for the support of long-term and basic research. Personal views are offered on how best to prepare and motivate the next generation of scientists in the field, and the need for scientists to engage in the debate of how embryo technologies should be used responsibly in countering global inequalities.
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http://dx.doi.org/10.1016/j.theriogenology.2005.09.005 | DOI Listing |
Arch Toxicol
January 2025
Department of Ecotoxicology, Helmholtz Centre for Environmental Research-UFZ, Permoserstraβe 15, 04318, Leipzig, Germany.
Testing for developmental toxicity is an integral part of chemical regulations. The applied tests are laborious and costly and require a large number of vertebrate test animals. To reduce animal numbers and associated costs, the zebrafish embryo was proposed as an alternative model.
View Article and Find Full Text PDFJ Assist Reprod Genet
January 2025
APHP Centre, Faculty of Medicine, Université Paris Cité, 75014, Paris, France.
Purpose: To evaluate if morphology and morphokinetics of cleaved embryos affect prenatal and perinatal outcomes.
Methods: This retrospective cohort study included 734 single fresh embryo transfer (SET) from ICSI from January 2014 to December 2020 at the Dijon University Hospital. Using time-lapse technology, embryos were defined as TOP or non-TOP according to morphological/morphokinetic criteria.
J Extracell Vesicles
January 2025
State Key Laboratory of Reproductive Medicine and offspring health, Nanjing Medical University, Nanjing, China.
As functional derivatives of mesenchymal stem cells (MSCs), small extracellular vesicles (sEVs) have garnered significant attention and application in regenerative medicine. However, the technical limitations for large-scale isolation of sEVs and their heterogeneous nature have added complexity to their applications. It remains unclear if the heterogeneous sEVs represent different aspects of MSCs functions.
View Article and Find Full Text PDFF S Rep
December 2024
Reproductive Center, Medical Corporation Group Mio Fertility Clinic, Kuzumo-Minami, Yonago, Japan.
Objective: To investigate whether artificial removal of zona pellucida (ZP) at the pronuclear stage improves good-quality embryos and blastocyst development in patients with difficulty conceiving because of severe fragmentation in early-cleavage stage.
Design: Exploratory investigation.
Setting: Reproductive center.
F S Rep
December 2024
Department of Obstetrics and Gynecology, University of South Florida, Morsani College of Medicine, Tampa, Florida.
Objective: To compare pregnancy outcomes after single blastocyst embryo transfer among patients whose first autologous embryo transfer was either a fresh embryo transfer or a frozen embryo transfer (FET) after a freeze-all, in the absence of preimplantation genetic testing for aneuploidy (PGT-A).
Design: A multicenter retrospective cohort analysis.
Setting: National multicenter fertility practice.
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