AI Article Synopsis
- A new technique combines reverse transcription polymerase chain reaction (RT-PCR) with a water-in-oil (W/O) emulsion for improved RNA detection.
- Reverse transcription and initial thermal cycles occur in separate droplets before they are merged for additional PCR cycles.
- This innovative two-step process was successful in detecting a single RNA molecule.
Article Abstract
A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.
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| http://dx.doi.org/10.1263/jbb.99.293 | DOI Listing |
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