Cell culture in a closed nano-space.

J Biosci Bioeng

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Published: October 2005

The minimum size of a closed nano-space in which cells can survive was determined using 4-nl nanowells. One or two cells could divide in the nanowell. Our results suggest that the cell division activity in the nano-space is determined by the conflict between intercellular effects and consumption of substrates.

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http://dx.doi.org/10.1016/S1389-1723(04)00286-5DOI Listing

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