Arabidopsis thaliana can be a useful model plant in the field of plant molecular biology because it is a sole dicotyledonous plant whose genome sequence has been completely determined at the present time. To develop functional genomics of A. thaliana, a large number of transgenic plants must be produced. However, transformation of the plants requires wide space and long time. For these reasons, a highly efficient method has been desired to be developed. In order to save space, the transformation system was improved, based on a new technology for plant propagation using ceramic tubes. The new method using ceramic tubes facilitated to prepare plants with a uniform growth level. In addition, the colander procedure after infiltration, which is one of tedious procedures, could be omitted. The new system without soil usage could cut down the cleaning up after experiment.
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http://dx.doi.org/10.1016/S1389-1723(03)70140-6 | DOI Listing |
BMC Plant Biol
January 2025
Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, 810016, China.
Barley leaf stripe, a disease mainly caused by Pyrenophora graminea (P. graminea) infection, severely affects barley yield and quality and is one of the most widespread diseases in barley production. However, little is known about the underlying molecular mechanisms of leaf stripe resistance.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
Key Laboratory of Chinese Medicinal Resources Recycling Utilization of National Administration of Traditional Chinese Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Flowering is a critical step in the plant life cycle. Angelica sinensis (Oliv.) Diels is a medicinal crop whose root is a well-known herbal medicine used in Asia.
View Article and Find Full Text PDFNat Commun
January 2025
Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Mediator25 (MED25) has been ascribed as a signal-processing and -integrating center that controls jasmonate (JA)-induced and MYC2-dependent transcriptional output. A better understanding of the regulation of MED25 stability will undoubtedly advance our knowledge of the precise regulation of JA signaling-related transcriptional output. Here, we report that Arabidopsis MED16 activates JA-responsive gene expression by promoting MED25 stability.
View Article and Find Full Text PDFNat Commun
January 2025
School of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, NY, USA.
Zoologists have adduced morphological convergence among embryonic stages of closely related taxa, which has been called the phylotypic stage of embryogenesis. Transcriptomic analyzes reveal an hourglass pattern of gene expression during plant and animal embryogenesis, characterized by the accumulation of evolutionarily older and conserved transcripts during mid-embryogenesis, whereas younger less-conserved transcripts predominate at earlier and later embryonic stages. In contrast, comparisons of embryonic gene expression among different animal phyla describe an inverse hourglass pattern, where expression is correlated during early and late stages but not during mid-embryo development.
View Article and Find Full Text PDFPlant Physiol
January 2025
Key Laboratory of Soybean Biology of Ministry of Education China, Northeast Agricultural University, Harbin 150030, China.
Plant height is a crucial agronomic characteristic that substantially influences soybean [Glycine max (L.) Merr.] yield.
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