Immunocytolocalization experiments indicate that carbonic anhydrase, phosphoribulokinase, and P-glycerate kinase are near neighbors of Rubisco in the pea leaf chloroplast stroma. Direct transfer of ribulose bisphosphate and gaseous CO(2) from phosphoribulokinase and carbonic anhydrase to Rubisco, and direct transfer of P-glycerate from Rubisco to P-glycerate kinase in the chloroplast stroma, is then a possibility. Rubisco activase, responsible for the removal of inhibitory sugar phosphates that bind to the active site of Rubisco in the dark, also appears to be co-localized with Rubisco.
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http://dx.doi.org/10.1023/B:PRES.0000040443.92346.37 | DOI Listing |
Plants (Basel)
October 2024
Federal State Institution Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, 119071 Moscow, Russia.
In addition to leaves, photosynthesis can occur in other green plant organs, including developing seeds of many crops. While the majority of studies examining photosynthesis are concentrated on the leaf level, the role of other green tissues in the production of total photoassimilates has been largely overlooked. The present work studies the photosynthetic behavior of leaves and non-foliar (pericarps, coats, and cotyledons) organs of pea ( L.
View Article and Find Full Text PDFJ Plant Res
November 2024
Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
The widespread use of antibiotics in intensive animal husbandry, and the agricultural utilization of manure from such farms, imposes a significant burden on the environment. Consequently, the effects of antibiotics should be studied not only in animals and humans but also in all components of biocenoses and agrocenoses. In our study, we analyze the impact of four different concentrations of tetracycline present in soil (0, 5, 50, and 500 mg/kg of soil) on the growth and key photosynthesis parameters of pea seedlings: chlorophyll concentration, aminolevulinic acid concentration, aminolevulinic acid dehydrogenase activity, and ribulose bisphosphate carboxylase-oxygenase (RuBisCO) activity.
View Article and Find Full Text PDFFood Funct
May 2024
Univ. Montpellier, INRAE, Institut Agro, IATE, Montpellier, France.
Growing demand for sustainable, plant-based protein sources has stimulated interest in new ingredients for food enrichment. This study investigates the nutritional and digestive implications of enriching wheat dough with RuBisCO, in comparison to pea protein-enriched and gluten-enriched doughs. The protein quality and digestibility of these enriched doughs were analysed through dough characterization, digestion experiments and biochemical analysis of digesta.
View Article and Find Full Text PDFJ Biosci
January 2024
Plant Molecular Stress Physiology Research Group, Department of Life Sciences, School of Life Sciences, Central University of Tamil Nadu, Thiruvarur 610005, India.
Drought stress affects photosynthesis, leading to significant decrease in crop productivity. In the current study, the importance of the cytochrome oxidase (COX) and alternative oxidase (AOX) pathways of themitochondrial oxidative electron transport chain (mETC) for photosynthesis and reactive oxygen species (ROS) homeostasis was evaluated in the leaves of plants exposed to drought stress for 3 days (D3), 6 days (D6), and 9 days (D9). While drought stress resulted in decreased CO assimilation rates, leaf stomatal conductance, transpiration, and leaf intercellular CO concentration in a stress-dependent manner, superimposition with mETC inhibitors, antimycin A (AA) and salicylhydroxamic acid (SHAM), aggravated the responses.
View Article and Find Full Text PDFPlants (Basel)
September 2023
Department of Biophysics, N. I. Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia.
Photodamage of photosynthetic electron transport is a key mechanism of disruption of photosynthesis in plants under action of stressors. This means that investigation of photodamage is an important task for basic and applied investigations. However, its complex mechanisms restrict using experimental methods of investigation for this process; the development of mathematical models of photodamage and model-based analysis can be used for overcoming these restrictions.
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