AI Article Synopsis
- A study focused on a mutant strain of Sinorhizobium fredii that causes pseudonodules on soybean plants and found that adding certain compounds did not restore effective symbiosis.
- Researchers constructed a deletion mutant in the purL gene to analyze its role in symbiosis with soybean, using various promoters to modify gene expression.
- Results showed that reduced or specific expression of the purL gene could successfully establish symbiosis with soybean without needing additional nutrients, but a minimal level of purL expression is still necessary for successful interaction.
Article Abstract
Previous reports showed that a transposon-induced PurL- mutant of Sinorhizobium fredii induced pseudonodules on Glycine max and the addition of 5-aminoimidazole-4-carboxamide-riboside or adenine to the plant could not restore the mutant to establish effective symbiosis. To gain a better understanding of the impact of the purL gene on symbiosis formation, we measured the effect of modified expression of this gene on the symbiotic abilities of S. fredii on soybean (G. max). A 1.98-kb in-frame deletion mutant in the purL gene of S. fredii was constructed. Transcriptional modification of the purL gene was conducted using several promoters such as those of lac, nifH, nifQ, and fixN. It was found that reduced expression of purL gene or suitable symbiotic expression of purL (such as with the promoter nifH or nifQ) can efficiently establish symbiosis of S. fredii on G. max without the exogenous supplementation of any adenine or purine precursor; at least a minimal level of expression of purL is essential for effective symbiosis with soybean.
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| http://dx.doi.org/10.1007/s00253-005-0186-4 | DOI Listing |
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