Comparison between 400 nt of mouse DNA sequence immediately upstream (5') of the coding sequence of the Sertoli cell expressed genes, Amh and Tsx, identified a 33 nt sequence with a significant identity: this was considered to be a candidate Sertoli specific regulatory element (SSRE). Another highly conserved sequence has been identified immediately downstream (3') of the Amh polyadenylation signal (DSRE). The action and specificity of these two putative regulatory elements, inserted into a reporter vector (pd2EGFP), has been investigated by transient transfection of mouse fibroblast and Sertoli cells. When combined together or alone, with a minimal thymidine kinase (Tk) promoter, SSRE showed a weak incremental action on the expression of the reporter gene, regardless of the cell type: in contrast there was a 2- to 3-fold decrease when DSRE was present. However, in the absence of Tk there was evidence for a strong synergy between SSRE and DSRE, which was significantly greater in the Sertoli as compared with the fibroblast cells. These results support the view that SSRE exhibits a degree of Sertoli specificity and acts synergistically with DSRE in controlling the expression of Amh.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gene.2005.08.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Improvement of Late-Onset Hypogonadism Symptoms of Fermented Extract in TM3 Leydig and TM4 Sertoli Cells.
Nutrients
November 2024
College of Pharmacy, Yonsei University, Songdogwahak-ro, Yeonsu-gu, Incheon 21983, Republic of Korea.
Background/objectives: Late-onset hypogonadism (LOH), characterized by declining testosterone levels with age, negatively affects the health of men, causing physical, psychological, and sexual dysfunction. Conventional testosterone replacement therapies have side effects, which has led to interest in natural alternatives. We investigated the effects of a standardized fermented extract (FME) on oxidative stress-induced damage in TM3 Leydig and TM4 Sertoli cells.
View Article and Find Full Text PDFIdentification of differentially expressed genes in human testis biopsies with defective spermatogenesis.
Reprod Med Biol
December 2024
Institute for Veterinary Anatomy, Histology and Embryology Justus-Liebig University of Giessen Giessen Germany.
Purpose: Sperm morphology and motility are major contributors to male-factor infertility, with many genes predicted to be involved. This study aimed to elucidate differentially expressed transcripts in human testis tissues of normal and abnormal spermatogenesis that could reveal new genes that may regulate sperm morphology and function.
Methods: Human testis biopsies were collected from men with well-characterized phenotypes of normal spermatogenesis, spermatid arrest, and Sertoli cell-only phenotype, and transcriptional differences were quantified by RNA-sequencing (RNA-Seq).
Postnatal Ovarian Transdifferentiation in the Absence of Estrogen Receptor Signaling Is Dependent on Genetic Background.
Endocrinology
November 2024
Reproductive & Developmental Biology Laboratory, NIEHS, NIH, Research Triangle Park, NC 27709, USA.
Normal ovarian function requires the expression of estrogen receptors α (ESR1) and β (ESR2) in distinct cell types within the ovary. The double estrogen receptor knockout (αβERKO) ovary had the appearance of seminiferous tubule-like structures that expressed SOX9; this phenotype was lost when the animals were repeatedly backcrossed to the C57BL/6J genetic background. A new line of ERKO mice, Ex3αβERKO, was developed for targeted disruption on a mixed genetic background.
View Article and Find Full Text PDFPotential of mesenchymal stromal/stem cells and spermatogonial stem cells for survival and colonization in bull recipient testes after allogenic transplantation.
Theriogenology
December 2024
Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, 8820808, Santiago, Chile; Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal, Facultad de Ciencias Biológicas y Facultad de Medicina, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Macul, 7820436, Santiago, Chile. Electronic address:
Stem cell transplantation into seminiferous tubules of recipient testis could become a tool for fertility restoration, genetic improvement, or conservation of endangered species. Spermatogonial stem cells (SSCs) are primary candidates for transplantation; however, limited abundance, complexity for isolation and culture, and lack of specific markers have limited their use. Mesenchymal stromal/stem cells (MSCs) are multipotent progenitors that are simple to isolate and culture and possess specific markers for identification, and immune evasive and migratory capacities.
View Article and Find Full Text PDFNew Insights into D-Aspartate Signaling in Testicular Activity.
Cells
August 2024
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', 81100 Caserta, Italy.
D-aspartate (D-Asp) is an amino acid found in high concentrations in the testis and pituitary gland. Increasing evidence suggests that D-Asp promotes spermatogenesis by activating testosterone production in the Leydig cells via LH release from the pituitary gland. In vitro studies indicate that D-Asp may also influence steroidogenesis and spermatogenesis through autocrine and paracrine signals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!