Phenotypic characteristics and results of PCR tests for the presence of species-specific genes indicate that a number of strains of thermophilic lactic acid bacteria previously considered as belonging to Streptococcus thermophilus are actually closely related to enterococci. In the present study, partial (over 500 nucleotides) sequencing of 16S rRNA genes from 12 strains of thermophilic lactic acid bacteria used as starters for manufacturing sour milk products on the territory of the Commonwealth of Independent States (CIS) has been performed. According to the results of the sequencing, seven of the strains have been classified with Enterococcus durans. The earlier classification (based on PCR tests) of two of the strains as S. thermophilus and three of the strains as E. faecium has been confirmed. The data obtained demonstrate that the enterococci E. durans and E. faecium are widely used as thermophilic starters for manufacturing sour milk products on the territory of the CIS.
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Bioresour Technol
January 2025
This study investigates the production of polyhydroxybutyrate (PHB) using the thermophilic bacterium Caldimonas thermodepolymerans in fed-batch fermentation. This research highlights the potential of thermophilic bacteria in biopolymer production due to their ability to operate at high temperatures, which reduces contamination risks and enhances energy efficiency. Optimal fermentation conditions were identified at a temperature of 50 °C, with the strain achieving a maximum specific growth rate (μ) of 0.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
January 2025
Department of Bioengineering, Faculty of Engineering and Natural Sciences, Istanbul Medeniyet University, Istanbul, 34700, Turkey.
Thermomonas hydrothermalis, a thermophilic bacterium isolated from hot springs, exhibits unique genomic features that underpin its adaptability to extreme environments and its potential in industrial biotechnology. In this study, we present a comparative genomic analysis of two strains, DSM 14834 and HOT.CON.
View Article and Find Full Text PDFMicroorganisms
December 2024
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
sp. THPS1 is a novel strain isolated from a high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed to characterize the genomic and functional attributes of sp.
View Article and Find Full Text PDFMicroorganisms
November 2024
The Italian Committee of the International Dairy Federation, 20131 Milano, Italy.
Natural whey starter (NWS) is an undefined complex culture used in the production of Grana Padano and Parmigiano Reggiano PDO cheeses. The aim of this review is to discuss, in light of the latest research results, the role of NWS as a primary player in the cheese-making process, considering the microbial community scenario. NWS is traditionally produced by fermenting part of the whey collected at the end of a previous cheese-making process.
View Article and Find Full Text PDFBMC Biotechnol
January 2025
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Background: In this study, thermophilic pectinase-producing strains were isolated. Among all the isolates, strain No. 4 was identified as Aspergillus fumigatus BT-4 based on its morphology and 18 S rDNA analysis.
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