AI Article Synopsis
- The research focuses on the expression and purification of neurotensin receptors, which are essential for structural studies like nuclear magnetic resonance.
- The methods involve using Escherichia coli to achieve functional expression of the receptor protein.
- An automated two-column purification process is utilized to facilitate continuous crystallization experiments and investigations on ligands that bind to the receptor.
Article Abstract
Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10969-005-1917-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Role of Pyroptosis-Related Gene Signature and Immune Infiltration in Juvenile Dermatomyositis.
Clin Cosmet Investig Dermatol
January 2025
Department of Clinical Laboratory, Central Hospital of Dalian University of Technology, Dalian, 116033, People's Republic of China.
Objective: Juvenile dermatomyositis (JDM) is a complex autoimmune disease, and its pathogenesis remains poorly understood. Building upon previous research on the immunological and inflammatory aspects of JDM, this study aims to investigate the role of pyroptosis in the pathogenesis of JDM using a comprehensive bioinformatics approach.
Methods: Two microarray datasets (GSE3307 and GSE11971) were obtained from the Gene Expression Omnibus database, and a list of 62 pyroptosis-related genes was compiled.
Exploring Tetraselmis chui microbiomes-functional metagenomics for novel catalases and superoxide dismutases.
Appl Microbiol Biotechnol
January 2025
Department of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
The focus on microalgae for applications in several fields, e.g. resources for biofuel, the food industry, cosmetics, nutraceuticals, biotechnology, and healthcare, has gained increasing attention over the last decades.
View Article and Find Full Text PDFMajor depressive disorder (MDD) is a common mood condition affecting multiple brain regions and cell types. Changes in astrocyte function contribute to depressive-like behaviors. However, while neuronal mechanisms driving MDD have been studied in some detail, molecular mechanisms by which astrocytes promote depression have not been extensively explored.
View Article and Find Full Text PDFImmobilization of Glycosyltransferase into a Hydrophilic Metal-organic Framework for Efficient Biosynthesis of Chondroitin Sulfate.
J Biotechnol
January 2025
Analysis and Testing Center, Nanjing Normal University, Nanjing, 210023, China. Electronic address:
Chondroitin sulfate (CS) is a structurally complex anionic polysaccharide widely used in medical, cosmetic and food applications. Enzymatic catalysis is an important strategy for synthesizing CS with uniform chain lengths and well-defined structures. However, the industrial application of glycosyltransferases is hindered by limitations such as low expression yields, poor stability, and challenges in reuse.
View Article and Find Full Text PDFProtocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays.
STAR Protoc
January 2025
Department of Molecular Medicine, University of Pavia, Pavia, Italy. Electronic address:
Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. Here, we present a protocol to express and purify milligram amounts of recombinant VDAC1 in Escherichia coli. We detail steps for a fluorescence polarization-based high-throughput screening assay using NADH displacement, along with procedures for thermostability, fluorescence polarization, and X-ray crystallography.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!