Mycobacterium tuberculosis infections are a major global health problem. Fast and accurate detection of M. tuberculosis is clearly needed at both the clinical level and the research level. We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1248482 | PMC |
| http://dx.doi.org/10.1128/JCM.43.10.5369-5371.2005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The 'genetic zipper' method offers a cost-effective solution for aphid control.
Front Insect Sci
December 2024
Department of General Biology and Genetics, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol, Republic of Crimea.
Twenty years ago, it was difficult to imagine the use of nucleic acids in plant protection as insecticides, but today it is a reality. New technologies often work inefficiently and are very expensive; however, qualitative changes occur during their development, making them more accessible and work effectively. Invented in 2008, contact oligonucleotide insecticides (olinscides, or DNA insecticides) based on the CUAD (contact unmodified antisense DNA) platform have been substantially improved and rethought.
View Article and Find Full Text PDFDevelopment and application of a quadruplex real-time fluorescence quantitative PCR assay for four porcine digestive pathogens.
Front Cell Infect Microbiol
December 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Introduction: , , , and are the primary pathogens responsible for gastrointestinal diseases in pigs, posing a significant threat to the health and productivity of pig production systems. Pathogen detection is a crucial tool for monitoring and managing these infections.
Methods: We designed primers and probes targeting the gene of , the 23S gene of , the gene of , and the gene of .
Nuclear eDNA metabarcoding primers for anthozoan coral biodiversity assessment.
PeerJ
December 2024
Lehigh Oceans Research Center, Lehigh University, Bethlehem, PA, United States.
The distributions of anthozoan corals are undercharacterized due to their wide bathymetric ranges, occurrences in remote locales, and difficulties of identification from morphology alone. Environmental DNA (eDNA) sequencing promises to be a noninvasive strategy to complement conventional approaches for mapping and monitoring the distribution and biodiversity of coral communities. Primers for eDNA metabarcoding have been designed to amplify nuclear and mitochondrial DNA barcodes in shallow scleractinians and mitochondrial in deep-sea octocorals.
View Article and Find Full Text PDFRapid Enzymatic Detection of Shiga-Toxin-Producing Using Fluorescence-Labeled Oligonucleotide Substrates.
ACS Infect Dis
December 2024
Department for Infectious Diseases, Division of Enteropathogenic Bacteria and Legionella (FG11), National Reference Centre for Salmonella and other Enteric Bacterial Pathogens, Robert Koch Institute, 38855 Wernigerode, Germany.
Article Synopsis
- Shiga-toxin-producing E. coli (STEC) are harmful bacteria that can cause severe illnesses like diarrhea and kidney failure, making quick detection crucial but difficult.
- A new rapid test has been developed using synthetic DNA substrates that produce a fluorescent signal when cleaved by Shiga toxins, allowing for easier identification of STEC.
- In testing 94 clinical strains, including various STEC serotypes, this assay proved effective for immediate detection of Shiga toxins, potentially enhancing outbreak response and treatment strategies.
A New Multiplex PCR Assay Reveals the Occurrence of E. bangladeshi alongside E. histolytica and E. moshkovskii in Eastern India.
Acta Parasitol
December 2024
Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India.
Purpose: Epidemiological studies on amoebic infections are complicated due to morphologically identical and clinically important Entamoeba species. Therefore, newer, simpler, and more economical diagnostic techniques are required for differentiating clinically important Entamoeba species.
Methods: We developed a single-round multiplex PCR assay to identify E.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!