We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1248492 | PMC |
| http://dx.doi.org/10.1128/JCM.43.10.5366-5368.2005 | DOI Listing |
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