We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1248459PMC
http://dx.doi.org/10.1128/JCM.43.10.5362-5365.2005DOI Listing

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