The recovery of Clostridium difficile from the stools of patients with C. difficile-associated diarrhea was evaluated by use of an enrichment broth (cycloserine-cefoxitin fructose broth supplemented with 0.1% sodium taurocholate [TCCFB]) and was compared to that from selective agar (cycloserine-cefoxitin fructose agar [CCFA]) and alcohol shock followed by inoculation onto blood agar (AS-BA). TCCFB was superior to CCFA and AS-BA, and neither the storage time nor the storage temperature affected the recovery rate.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1248507 | PMC |
http://dx.doi.org/10.1128/JCM.43.10.5341-5343.2005 | DOI Listing |
Microbiol Resour Announc
January 2025
Singapore Centre for Environmental Life Sciences Engineering (SCELSE), National University of Singapore, , Singapore.
Coastal water, sediment, and algae samples were collected from St. John's Island, Singapore, and enriched in either broth or agar. Metagenomic sequencing was carried out on DNA from these enrichments and analyzed.
View Article and Find Full Text PDFFoods
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
This study aimed to assess the impact of radish sprouts on the gut microbiota of healthy individuals. Radish sprout additives, subjected to short-term storage and steam treatment, were used to intervene in an in vitro culture of human gut microbiota. The influence of radish sprouts on the gut microbiota was evaluated by monitoring short-chain fatty acid (SCFA) content and proportion in the fermentation broth, and microbial diversity was assessed using 16S rDNA amplicon sequencing.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
Department of Veterinary Science, Martin-Gatton College of Agriculture, Food, and Environment, University of Kentucky, Lexington, Kentucky, USA.
Unlabelled: is a foodborne pathogen that poses a significant threat to global public health. It affects several animal species, including horses. infections in horses can be either asymptomatic or cause severe clinical illness.
View Article and Find Full Text PDFFront Microbiol
January 2025
Department of Agriculture, Food and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States.
Metagenomic sequencing is increasingly being employed to understand the assemblage and dynamics of the oyster microbiome. Specimen collection and processing steps can impact the resultant microbiome composition and introduce bias. To investigate this systematically, a total of 54 farmed oysters were collected from Chesapeake Bay between May and September 2019.
View Article and Find Full Text PDFInt J Food Microbiol
January 2025
Department of Pathobiology and Population Sciences, Royal Veterinary College, NW1 0TU London, United Kingdom; Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark. Electronic address:
We determined the frequency, genotypes, phenotypes, and mobility of extended-spectrum β-lactamase (ESBL)-encoding genes in Enterobacteriaceae isolated from retail seafood products. Overall, 288 samples of fresh shrimps, catfish and seabass imported from Asia were collected from three supermarket chains in the UK (96 each). After enrichment in MacConkey broth supplemented with cefotaxime, total DNA was screened for the presence of CTX-M, SHV and TEM by real-time PCR.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!