Identification of botanicals and potential contaminants through RFLP and sequencing.

Planta Med

Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095-1606, USA.

Published: September 2005

Botanical supplements for health enhancement are being increasingly used in the United States, but no safeguards are formally in place to ensure that they are not contaminated with non-efficacious or potentially harmful plant material. A molecular approach, which allows the authentication of botanical ingredients and detection of contaminating plant material by analyzing the ITS-1 region by PCR-RFLP and subsequent sequencing, is described. When using starting material from which DNA can be obtained, this method has the potential for identifying both primary and contaminating plant material in botanical dietary supplements.

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-2005-871230DOI Listing

Publication Analysis

Top Keywords

plant material
12
contaminating plant
8
identification botanicals
4
botanicals potential
4
potential contaminants
4
contaminants rflp
4
rflp sequencing
4
sequencing botanical
4
botanical supplements
4
supplements health
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!