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Brominated skeletal components of the marine demosponges, Aplysina cavernicola and Ianthella basta: analytical and biochemical investigations.
Mar Drugs
April 2013
General Biochemistry, TU Dresden, Dresden 01062, Germany.
Demosponges possess a skeleton made of a composite material with various organic constituents and/or siliceous spicules. Chitin is an integral part of the skeleton of different sponges of the order Verongida. Moreover, sponges of the order Verongida, such as Aplysina cavernicola or Ianthella basta, are well-known for the biosynthesis of brominated tyrosine derivates, characteristic bioactive natural products.
View Article and Find Full Text PDFProteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
J Biol Chem
November 2002
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures that resist Triton extraction, and by attachments to underlying cytoskeletal proteins in assemblies called "membrane skeletons." Although the actin cytoskeleton is implicated in many lipid raft-mediated signaling processes, little is known about the biochemical basis for actin involvement. We show here that a subset of plasma membrane skeleton proteins from bovine neutrophils co-isolates with cholesterol-rich, detergent-resistant membrane fragments (DRMs) that exhibit a relatively high buoyant density in sucrose (DRM-H; d approximately 1.
View Article and Find Full Text PDFRegulation of von Willebrand factor binding to the platelet glycoprotein Ib-IX by a membrane skeleton-dependent inside-out signal.
J Biol Chem
May 2001
Department of Pharmacology, University of Illinois, College of Medicine, Chicago, Illinois 60612, USA.
The platelet receptor for von Willebrand factor (vWF), glycoprotein Ib-IX (GPIb-IX), mediates initial platelet adhesion and activation. We show here that the receptor function of GPIb-IX is regulated intracellularly via its link to the filamin-associated membrane skeleton. Deletion of the filamin binding site in GPIb(alpha) markedly enhances ristocetin- (or botrocetin)-induced vWF binding and allows GPIb-IX-expressing cells to adhere to immobilized vWF under both static and flow conditions.
View Article and Find Full Text PDFIsolation of skeleton-associated knobs from human red blood cells infected with malaria parasite Plasmodium falciparum.
Mol Biochem Parasitol
June 1992
Department of Biomedical Research, St. Elizabeth's Hospital, Tufts University School of Medicine, Boston, MA 02135.
Isolation and characterization of stable nuclear matrix preparations and associated DNA from avian erythroblasts.
Biol Cell
March 1989
Institut Jacques Monod (CNRS), Université Paris VII, France.
A novel procedure for isolation of nuclear matrices from chicken erythroblast cells was elaborated. The influence of variations in the isolation procedure on structural integrity and morphology of nuclear matrices as well as on properties of the nuclear matrix-associated DNA fractions was investigated. The incubation of isolated nuclei in the presence of Cu2+ ions provided significant stabilization of the nuclear matrix.
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