Objective: To express and purify the HIV p24 gene in E. coli cells, and identify p24 antigen activity.
Methods: The full length gene fragment of HIV p24 was amplified by PCR and inserted into the pRSET vector in order to construct the pRSET-p24 recombined vector. After transforming into E. coil, the purified p24 protein was prepared by metal-ligand affinity chromatography (IMAC). The accuracy of inserted gene and activity, specificity of HIV p24 proteins were detected by two enzymes digestion technology, SDS-PAGE, Western Blot (WB) and ELISA.
Results: The length of the HIV p24 gene fragment was 690 bp after digesting the recombinant plasmid T-p24 and pRSET-p24 with BamH I and Hind III. The expressed proteins had a single expected band of about 24 x 10(3) in SDS-PAGE. The specificity and activity of p24 protein were tested by WB and ELISA.
Conclusion: The HIV p24 sequence from HIV-1 gene plasmid hasbeen expressed in E. coil. This protein possessed good specificity and activity.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Comparison: Imported and Indigenous HIV Fourth-generation immunodiagnostic kits.
Asian J Transfus Sci
December 2024
Immunodiagnostic Kit Laboratory, National Institute of Biologicals, Noida, Uttar Pradesh, India.
Early detection of any disease is always a life-saving methodology for human beings likewise the detection of p24 antigen is always better than the detection of HIV antibodies. In the current era, p24 antigen is added additionally in HIV antibodies detection assays and called HIV fourth-generation immunodiagnostic kit. The different HIV fourth-generation kit having different capacities to pick up the low concentration of analyte as well as represent the detection values in different units.
View Article and Find Full Text PDFAnalysis of HIV infection among voluntary blood donors based on HIV ELISA and nucleic acid detection.
Am J Transl Res
December 2024
Blood Transfusion Laboratory, Jiangxi Provincial Blood Center Nanchang 330052, Jiangxi, China.
Objective: To analyze the epidemiological characteristics of human immunodeficiency virus (HIV) infection among voluntary blood donors and provide a foundation for improving the donor recruitment strategies and developing a more scientific and effective HIV screening strategy.
Methods: HIV testing data from voluntary blood donors in Nanchang, collected from January 2021 to February 2024, were analyzed. According to the serologic and nucleic acid sequence detection mode, two different reagents were used for ELISA detection and NAT detection.
Background: Due to the unique geographical and climatic conditions in Nagqu (Tibet), the blood station laboratory was only fully established and accredited by 2020. This study validated the performance of the laboratory's blood screening system and analyzed recent trends in blood donation and screening effectiveness.
Methods: Various serum samples were used to assess the performance of hepatitis B, hepatitis C, HIV, and syphilis tests, both serological and nucleic acid tests.
The feasibility and impact of deploying a four-tests panel at antenatal care in primary health care facilities of a developing country, Kenya.
Front Public Health
December 2024
Centre for Biotechnology Research Development (CBRD), Kenya Medical Research Institute (KEMRI), Nairobi, Kenya.
Introduction: Contracting HIV, syphilis, hepatitis B virus (HBV), and malaria during pregnancy significantly affects the health of the woman, the pregnancy, and the unborn child. The World Health Organization (WHO) recommends testing pregnant women for these infections to achieve triple elimination of mother-to-child transmissions. However, this goal has not been fully realized in low- to medium-income countries, primarily due to segmented testing practices.
View Article and Find Full Text PDFMitochondrial resilience and antioxidant defence against HIV-1: unveiling the power of extracts and Shatavarin IV.
Front Microbiol
October 2024
Division of Virology, ICMR - National Institute of Translational Virology and AIDS Research, Pune, India.
Article Synopsis
- AR, an Ayurvedic botanical, was studied for its effects on HIV-1 replication and its ability to reduce mitochondrial dysfunction during infection.
- Both AR root extracts and its main active compound, Shatavarin IV, showed non-toxic, dose-dependent inhibition of HIV-1 in laboratory tests, particularly effective against certain viral subtypes.
- Shatavarin IV interacted with key enzymes involved in HIV-1 replication, suggesting its potential as a therapeutic agent, while also helping to alleviate mitochondrial damage caused by the virus.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!