The DNA polymerase reaction by Klenow fragment (KF) was efficiently regulated with UV light using a 25-mer caged fluorescent oligodeoxynucleotide (CFO) as the template. The CFO was functionalized with a fluorescein reporter (Fl) and photocleavable DABSYL quencher moiety (Dab). With Fl and Dab at adjacent cytidines in the middle at the template, KF was blocked from extending a complementary 12-mer primer. Upon UV photolysis of the DABSYL blocking group under aerobic conditions, fluorescein emission was restored and 50% of the primers were fully extended by KF.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bmcl.2005.08.058 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Analysis of circulating cell-free nuclear and mitochondrial DNA in plasma of Mexican patients with breast cancer.
Gac Med Mex
January 2025
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara.
Background: The usefulness of circulating free DNA (cfDNA), nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) as potential biomarkers in cancer remains controversial.
Objective: To determine the concentration of cfDNA and plasma nDNA and mtDNA levels in breast cancer (BC) patients.
Material And Methods: This study included a total of 86 women (69 patients with BC and 17 women as a control group).
Molecular cloning, sequencing, expression and purification of Alkhumra hemorrhagic fever virus capsid protein in Saudi Arabia.
J Infect Dev Ctries
December 2024
Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia.
Introduction: Alkhumra hemorrhagic fever virus (AHFV) is a newly discovered virus in the Flaviviridae family. It was discovered in 1995 among animal handlers in Saudi Arabia. AHFV spreads through close contact with infected animals and tick bites.
View Article and Find Full Text PDFMolecular evidence of (Pallas, 1781) in cockchafers in rural areas of Elazig, Türkiye.
J Helminthol
January 2025
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Türkiye.
The primary definitive host of the giant acanthocephalan, also known as the giant thorny-headed worm (Pallas, 1781), is . The definitive host ingests the parasite by consuming infected scarabaeoid or hydrophilid beetles. This study aimed to ascertain the presence of in the intermediate hosts through molecular analysis.
View Article and Find Full Text PDFSingle-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide.
Nucleic Acids Res
January 2025
Department of Health Sciences and Technology, ETH Zurich, Schmelzbergstrasse 9, Zurich 8092, Switzerland.
Temozolomide kills cancer cells by forming O6-methylguanine (O6-MeG), which leads to cell cycle arrest and apoptosis. However, O6-MeG repair by O6-methylguanine-DNA methyltransferase (MGMT) contributes to drug resistance. Characterizing genomic profiles of O6-MeG could elucidate how O6-MeG accumulation is influenced by repair, but there are no methods to map genomic locations of O6-MeG.
View Article and Find Full Text PDFPrevalence of Site-Specific Infection and Macrolide and Fluoroquinolone-Associated Mutations in Men Who Have Sex with Men in Shenzhen, China.
Infect Drug Resist
January 2025
School of Public Health, Fudan University, Shanghai, People's Republic of China.
Background: (MG) poses a growing public health concern due to the escalating antimicrobial resistance. We aimed to assess site-specific MG infection and its correlates and macrolide and fluoroquinolones mutations among men who have sex with men (MSM) in Shenzhen, China.
Methods: Samples were obtained from different anatomic sites of MSM based on their sexual behavior.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!