Synchrotron-radiation-based computer microtomography (SRmicroCT) was applied to three biomineralised objects First, embryonic snails of the freshwater snail Biomphalaria glabrata, second, rhopalia (complex sense organs) of the medusa Aurelia aurita, and third, human teeth. The high absorption contrast between the soft tissue and mineralised tissues, i.e. the shell in the first case (consisting of calcium carbonate) and the statoliths in the second case (consisting of calcium sulphate hemihydrate), makes this method ideal for the study of biomineralised tissues. The objects can be non-destructively studied on a micrometre scale, and quantitative parameters like the thickness of a forming a snail shell or statolith crystal sizes can be obtained on a length scale of 1-2 mum. Using SRmicroCT, the dentin-enamel border can be clearly identified in X-ray dense teeth.
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http://dx.doi.org/10.1007/s00775-005-0023-3 | DOI Listing |
J Synchrotron Radiat
January 2025
Cardiovascular Resarch Group iCare4Kids, Institut de Recerca Sant Joan de Déu, Esplugues de Llobregat, Spain.
One of the main limitations of conventional absorption-based X-ray micro-computed tomography imaging of biological samples is the low inherent X-ray contrast of soft tissue. To overcome this limitation, the use of ethanol as contrast agent has been proposed to enhance image contrast of soft tissues through dehydration. Some authors have shown that ethanol shrinks and hardens the tissue too much, also causing small tissue ruptures due to fast dehydration.
View Article and Find Full Text PDFSci Rep
October 2024
Lund University Diabetes Center, Lund University, Lund, Sweden.
Metabolic dysfunction-associated steatohepatitis (MASH) is a common but frequently unrecognized complication of obesity and type 2 diabetes. The association between these conditions is multifaceted and involves complex interactions between metabolic, inflammatory, and genetic factors. Here we assess the underlying structural and molecular processes focusing on the immunological phase of MASH in the nonobese inflammation and fibrosis (NIF) mouse model and compare it to the human disease as well as other murine models.
View Article and Find Full Text PDFBioact Mater
November 2024
Institute of Metallic Biomaterials, Helmholtz Zentrum Hereon, Geesthacht, Germany.
J Struct Biol
September 2024
Univ Lyon, INSA Lyon, Universite Claude Bernard Lyon 1, UJM-Saint Etienne, CNRS UMR 5220, Inserm U1206, CREATIS, 69621 Lyon, France.
Biotechnol Bioeng
October 2024
School of Life Sciences, Process Systems Engineering, Technical University of Munich, Freising, Germany.
Controlling the morphology of filamentous fungi is crucial to improve the performance of fungal bioprocesses. Microparticle-enhanced cultivation (MPEC) increases productivity, most likely by changing the fungal morphology. However, due to a lack of appropriate methods, the exact impact of the added microparticles on the structural development of fungal pellets is mostly unexplored.
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