The LD50, median survival time (ST50) and virus production are virulence parameters that are commonly used to describe the biological characteristics of viruses. In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 and -MCp5) that carry Tc1-like insect transposable elements. The three virus genotypes were similar in their LD50, ST50 and virus production. However, the mutant genotypes MCp4 and MCp5 were very effectively out-competed by CpGV-M in direct competition experiments, where Cydia pomonella larvae were co-infected with known ratios of occlusion bodies or budded virus of CpGV-M and one of the two mutants. It was demonstrated that MCp5 and MCp4 could not be sustained in the virus population when the progeny viruses of different co-infections were used as inocula to infect next passage larvae. These results show that the virulence parameters LD50, ST50 and virus production alone do not adequately reflect the competitiveness of the virus and are thus not suitable to describe virus population dynamics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/vir.0.81152-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CRISPR/Cas9-mediated knockout of Tektin 4-like gene (TEKT4L) causes male sterility of Cydia pomonella.
Insect Biochem Mol Biol
January 2025
College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China; Key Laboratory of Economical and Applied Entomology of Liaoning Province, China; Key Laboratory of Major Agricultural Invasion Biological Monitoring and Control, Shenyang, 110866, Liaoning, China. Electronic address:
Article Synopsis
- The sterile insect technique (SIT) is an eco-friendly method used for controlling pest populations, highlighting the importance of identifying genes that regulate insect fertility.
- Researchers discovered seven TEKT genes in the fruit pest Cydia pomonella, with TEKT4L showing the highest expression in male testes, indicating its key role in reproduction.
- Using CRISPR/Cas9, they knocked out TEKT4L, inducing male sterility and significantly reducing the hatching rates of offspring, which ultimately proved effective in controlling C.pomonella populations in cage trials.
Assessing pest control treatments from phenology models and field data.
Pest Manag Sci
December 2024
Department of Entomology, Washington State University, Pullman, WA, USA.
Background: Efficacy of insecticides is often determined from apparent yield loses due to a target pest. However, pests can affect yields even when controls work as expected. Further, most pest populations are monitored through adult counts without procedures to assess dynamics of immature stages.
View Article and Find Full Text PDFLinalool fumigation improves mating competitiveness of males for population suppression of the global fruit pest Cydia pomonella.
Pest Manag Sci
November 2024
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Article Synopsis
- * Results showed that a dose of 200 Gy resulted in high male sterility but significantly decreased their mating competitiveness due to reduced expression of certain pheromone recognition genes.
- * The research suggests that using plant volatiles, like linalool, can enhance mating competitiveness in irradiated males, indicating a viable method to improve the effectiveness of sterile insect technique (SIT) in pest management.
Biocontrol Potential of the New Codling Moth Granulovirus (CpGV) Strains.
Microorganisms
September 2024
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
The use of CpGV strains as the basis for bioinsecticides is an effective and safe way to control . The research is aimed at the identification and study of new CpGV strains. Objects of identification and bioinformatic analysis: 18 CpGV strains.
View Article and Find Full Text PDFStability, Inheritance, Cross-Resistance, and Fitness Cost of Resistance to λ-Cyhalothrin in .
J Agric Food Chem
October 2024
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.
Insecticides are commonly utilized in agriculture and forestry for pest control, but their dispersal can pose hazards to humans and environment. Understanding resistance, inheritance patterns, and fitness costs can help manage resistance. A λ-cyhalothrin-resistant population (LCR) of , a global pest of pome fruits and walnuts, was obtained through selective insecticide breeding for 15 generations, showing stable moderate resistance (23.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!