AI Article Synopsis
- The study examined various microscopy techniques to measure the size of ultimate fibers from harakeke, a plant native to New Zealand.
- Key variables in the study included geographic location, time of year, and specific plant characteristics, while the experimental focus was on the microscopy methods used.
- Results showed that the most effective techniques were light microscopy of thin transverse leaf sections and scanning electron microscopy of nonfixed fibers.
Article Abstract
The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (Phormium tenax, New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual plant, a fourth leaf from a north-facing fan, age of plant, and cultivars (two). Experimental variables were microscopy techniques and measurement axis. Measurements of width and length of harakeke ultimate fibers depended on the microscopic preparation/technique used as well as the cultivar examined. The best methods were (i) transverse sections of leaf specimens 4 microm thick, embedded in Paraplast and observed using light microscopy, and (ii) nonfixed ultimate fibers observed using scanning electron microscopy.
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| http://dx.doi.org/10.1002/jemt.20206 | DOI Listing |
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