AI Article Synopsis
- Cry4Ba is a delta-endotoxin from Bacillus thuringiensis israelensis that is highly toxic to Aedes aegypti larvae but not to Culex quinquefasciatus larvae.
- Cyt2Aa2, another endotoxin from Bacillus thuringiensis darmstadiensis, shows moderate toxicity to both mosquito species.
- When both toxins are expressed together in Escherichia coli, their combined effect significantly increases toxicity to both Aedes aegypti and Culex quinquefasciatus, showing a strong synergistic effect.
Article Abstract
Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC(50) values of 350 and 250 ng ml(-1), respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti andC. quinquefasciatus larvae (LC(50)=7 and 20 ng ml(-1), respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae.
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| http://dx.doi.org/10.1016/j.femsle.2005.08.038 | DOI Listing |
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