Developments in the field of phosphoproteomics have been fueled by the need simultaneously to monitor many different phosphoproteins within the signaling networks that coordinate responses to changes in the cellular environment. This article presents a brief review of phosphoproteomics with an emphasis on the biological insights that have been derived so far.
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http://dx.doi.org/10.1186/gb-2005-6-9-230 | DOI Listing |
Int J Mol Sci
January 2025
Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Peanut ( L.) is one of the most important crops for oil and protein production. The unique characteristic of peanut is geocarpy, which means that it blooms aerially and the peanut gynophores (pegs) penetrate into the soil, driving the fruit underground.
View Article and Find Full Text PDFExpert Rev Proteomics
January 2025
Biozentrum, University of Basel, Basel, Switzerland.
Introduction: Recent work identified members of the evolutionarily conserved coronin protein family as key regulators of cell population size. This work originated ~25 years ago through the identification, by two-dimensional gel electrophoresis, of coronin 1 as a host protein involved in the virulence of . We here describe the journey from a spot on a 2D gel to the recent realization that coronin proteins represent key controllers of eukaryotic cell population sizes, using ever more sophisticated proteomic techniques.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Life Science, Henan Normal University, Xinxiang 453007, Henan Province, PR China. Electronic address:
Phosphorylation plays a crucial role in the cellular response to radiation and cancer therapies, yet phosphoproteomics studies in planarians remain underexplored despite the organism's remarkable regenerative capacities. This study utilized advanced ion mobility mass spectrometry for 4D-label-free quantitative proteomics to identify phosphorylation sites associated with irradiation in planarians. A total of 33,284 phosphorylation sites from 15,505 phosphorylated peptides and 4710 unique phosphoproteins were identified.
View Article and Find Full Text PDFACS Chem Neurosci
January 2025
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, United States.
Hibernating mammals such as the thirteen-lined ground squirrel () experience significant reductions in oxidative metabolism and body temperature when entering a state known as torpor. Animals entering or exiting torpor do not experience permanent loss of brain function or other injuries, and the processes that enable such neuroprotection are not well understood. To gain insight into changes in protein function that occur in the dramatically different physiological states of hibernation, we performed quantitative phosphoproteomics experiments on thirteen-lined ground squirrels that are summer-active, winter-torpid, and spring-active.
View Article and Find Full Text PDFExp Neurol
January 2025
Department of Military Traffic Injury Prevention and Control, Daping Hospital, Army Medical University, Chongqing, China. Electronic address:
Traumatic brain injury (TBI), particularly at high altitudes (HA-TBI), is a leading cause of mortality and disability, yet clear diagnostic and treatment protocols are lacking. This study explores the early pathophysiological changes occurring within 24 h following HA-TBI, with a focus on differentially expressed proteins (DEPs) and phosphorylated proteins (DEPPs). Using a low-pressure hypoxic chamber to simulate high-altitude conditions combined with a controllable cortical impact (CCI) model, we established a rat model of HA-TBI.
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