Amongst 440 strains of Pseudomonas stutzeri isolated from soil and marine sediment for a population genetic study, eight strains were each presumed to represent a novel genomic group and were compared with each other and to reference strains of P. stutzeri genomovars 1 to 10 and other Pseudomonas species by DNA-DNA hybridization, 16S rRNA and internally transcribed 16S-23S rRNA spacer region (ITS1) sequences and basic physiological properties defining the species. While 16S rRNA and ITS1 gene sequences positioned the eight strains within the phylogenetic branch of P. stutzeri, the DNA-DNA hybridizations with reference strains of the 10 described genomovars and among the novel strains were generally below 70 %, which is the threshold for species and genomovar differentiation. Since the physiological properties studied in the eight strains fitted the profile of P. stutzeri, eight new genomovars of P. stutzeri, numbered 11 to 18, are proposed, with strains 28a50, 28a39, 28a22, 28a3, 4C29, 24a13, 24a75 and MT-1 being the reference strains. The highly transformable reference strain 28a3 of genomovar 14 had a localized 16S rRNA gene sequence tag characteristic of genomovar strains 2 and 3, suggesting a possible horizontal gene transfer event involving part of the 16S rRNA gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/ijs.0.63535-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders.
Sci Rep
January 2025
Center of Elephant and Wildlife Health, Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Colic and diarrhea are common gastrointestinal (GI) disorders in captive Asian elephants, which can severely impact health and lead to mortality. Gut dysbiosis, indicated by alterations in gut microbiome composition, can be observed in individuals with GI disorders. However, changes in gut microbial profiles of elephants with GI disorders have never been investigated.
View Article and Find Full Text PDFInfluence of selected dosages of plastic microparticles on the porcine fecal microbiome.
Sci Rep
January 2025
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719, Olsztyn, Poland.
Studies conducted so far have shown that nano- and microplastic may disturb the intestinal microenvironment by interacting with the intestinal epithelium and the gut microbiota. Depending on the research model used, the effect on the microbiome is different-an increase or decrease in selected taxa resulting in the development of dysbiosis. Dysbiosis may be associated with intestinal inflammation, development of mental disorders or diabetes.
View Article and Find Full Text PDFAlgal-bacterial bioremediation of cyanide-containing wastewater in a continuous stirred photobioreactor.
World J Microbiol Biotechnol
January 2025
The Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
This study reports the isolation and characterization of highly resistant bacterial and microalgal strains from an Egyptian wastewater treatment station to cyanide-containing compounds. The bacterial strain was identified as Bacillus licheniformis by 16S rRNA gene sequencing. The isolate removed up to 1 g L potassium cyanide, 3 g L benzonitrile, and 1 g L sodium salicylate when incubated as 10% v/v in MSM at 30 ℃.
View Article and Find Full Text PDFExploration of Cytokines and Microbiome Among Males and Females with Diarrhea-Predominant Irritable Bowel Syndrome.
Dig Dis Sci
January 2025
School of Nursing, University of Washington, Seattle, WA, USA.
Background: Whether pathophysiological factors differ between males and females with irritable bowel syndrome-diarrhea (IBS-D) remains to be tested. To better understand potential sex differences, males with IBS-D were compared to naturally cycling females and to females with IBS-D taking hormonal contraception on plasma levels of cytokines and gut microbiome characteristics.
Methods: Males and females with Rome III IBS-D completed questionnaires and kept a daily symptom diary for 28 days.
New Strains of the Deep Branching Streptophyte Streptofilum: Phylogenetic Position, Cell Biological and Ecophysiological Traits, and Description of Streptofilum arcticum sp. nov.
Environ Microbiol
January 2025
Institute for Biological Sciences, Applied Ecology and Phycology, University Rostock, Rostock, Germany.
Streptofilum capillatum was recently described and immediately caught scientific attention, because it forms a phylogenetically deep branch in the streptophytes and is characterised by a unique cell coverage composed of piliform scales. Its phylogenetic position and taxonomic rank are still controversial discussed. In the present study, we isolated further strains of Streptofilum from biocrusts in sand dunes and Arctic tundra soil.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!