Objective: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae.
Method: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm.
Result: The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6).
Conclusion: The results showed that method was reliable and accurate.
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