By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236620 | PMC |
| http://dx.doi.org/10.1128/JB.187.18.6590-6595.2005 | DOI Listing |
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