AI Article Synopsis
- Allelic loss of chromosome 1p is commonly seen in oligodendrogliomas, with a study of 177 tumors identifying 6 with localized 1p36 deletions.
- The 1p36 region has several genes related to apoptosis regulation, including DFFB, which showed decreased expression in tumors with the 1p deletion compared to those without, based on quantitative reverse-transcriptase PCR analysis.
- Although no mutations were found in the DFFB gene, the loss of one copy due to 1p deletions might play a role in the development of oligodendroglioma.
Article Abstract
Allelic loss of chromosome 1p is frequently observed in oligodendroglioma. We screened 177 oligodendroglial tumors for 1p deletions and found 6 tumors with localized 1p36 deletions. Several apoptosis regulation genes have been mapped to this region, including Tumor Protein 73 (p73), DNA Fragmentation Factor subunits alpha (DFFA) and beta (DFFB), and Tumor Necrosis Factor Receptor Superfamily Members 9 and 25 (TNFRSF9, TNFRSF25). We compared expression levels of these 5 genes in pairs of 1p-loss and 1p-intact tumors using quantitative reverse-transcriptase PCR (QRTPCR) to test if 1p deletions had an effect on expression. Only the DFFB gene demonstrated decreased expression in all tumor pairs tested. Mutational analysis did not reveal DFFB mutations in 12 tested samples. However, it is possible that DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1242250 | PMC |
| http://dx.doi.org/10.1186/1476-4598-4-35 | DOI Listing |
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