AI Article Synopsis
- The study examined bacterial communities in different types of benthic detritus across three streams, focusing on how sediment and water chemistry influenced these communities.
- A new cell purification method was developed and tested using fluorescence in situ hybridization (FISH) techniques, comparing traditional probes with amplified detection methods like CARD-FISH.
- Findings indicated that prokaryote numbers varied with water chemistry in low organic carbon detritus, while higher organic content showed no such trend, suggesting a link between detritus quality and microbial community structure, which could aid in river ecosystem assessments.
Article Abstract
Bacterial communities associated with a variety of benthic detritus types were studied in three streams in the context of the chemical characteristics of the sediment material and the stream water. A cell purification assay was developed for a quantitative microscopic evaluation of bacterial community structure in detritus samples by fluorescence in situ hybridization (FISH). The efficiency of FISH with fluorescently monolabelled probes was compared with FISH with signal amplification by catalysed reporter deposition (CARD-FISH). In detritus types poor in organic carbon and nitrogen, the numbers of prokaryotes were related to the chemical characteristics of the stream water column, whereas no such relationship was found for detritus types rich in organic carbon and nitrogen. These results might help to provide criteria for the selection of detritus types for river ecosystem assessment and monitoring. The percentage of bacteria detected by FISH with monolabelled probes was correlated with the detritus total organic matter (OM). This is likely attributed to a higher ribosome content of microbial cells on substrates rich in OM. Cell detection by CARD-FISH did not show any correlation with OM content, indicating that this technique renders the results more independent from the activity state of cells. Fluorescence in situ hybridization with four group-specific probes suggested a relationship between substrate quality and the composition of the microbial assemblages on the various types of detritus. The improved protocol for cell purification and CARD-FISH may facilitate future investigations on the relationship between the riverine benthic detritus quality and microbial community composition.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1462-2920.2005.00857.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Larval competition between the invasive Aedes aegypti (Diptera: Culicidae) and the Caribbean endemic Aedes mediovittatus (Diptera: Culicidae) from Puerto Rico, USA.
J Med Entomol
December 2024
Entomology and Nematology Department, Florida Medical Entomology Laboratory, Institute of Food and Agricultural Sciences, University of Florida, Vero Beach, FL, 32962, USA.
Competition between mosquito species during the larval phase is a well-established mechanism structuring container mosquito communities, with invasive species often outperforming natives. We assessed the competitive outcome between 2 species that occur on the island of Puerto Rico, the historic invasive Aedes aegypti (L.) and the endemic Aedes mediovittatus (Coquillett) (Diptera: Culicidae).
View Article and Find Full Text PDFDiet-induced plasticity modifies relationships between larval growth rate and post-metamorphic behavior and physiology in spadefoot toads.
J Exp Biol
January 2025
Department of Biology, Indiana University at Bloomington, Bloomington, IN 47405-7005, USA.
It has frequently been hypothesized that among-individual variation in behavior and physiology will correlate with life history traits, yet the nature of these correlations can vary. Such variability may arise from plasticity in trait development, which can amplify or attenuate trait correlations across different environments. Using the Mexican spadefoot toad (Spea multiplicata), we tested whether relationships between larval growth rate and post-metamorphic behavior or physiology are influenced by a key mediator of developmental plasticity: larval diet type.
View Article and Find Full Text PDFDecomposition of Sargassum detritus varies with exposure to different plastic types.
Environ Sci Pollut Res Int
December 2024
National Marine Science Centre and Marine Ecology Research Centre, Southern Cross University, PO Box 4321, Coffs Harbour, NSW, 2450, Australia.
Plastic pollution and ocean warming threaten crucial ecosystem processes, including detrital decomposition. We carried out a manipulative experiment using 20 outdoor raceways to test hypotheses about the influence of macroplastics (polyvinyl chloride (PVC), polyethylene terephthalate (PET), high-density polyethylene (HDPE), low-density polyethylene (LDPE), and biodegradable (BIO)) and ocean warming (as 3 °C above ambient sea surface temperatures) on the decomposition of Sargassum vestitum. All types of plastic significantly decreased rates of S.
View Article and Find Full Text PDFArticle Synopsis
- The study addresses the challenge of accurately measuring particulate organic carbon (POC) in coastal waters due to the complex variability of marine particles.
- A new method was created that uses the ratio of phytoplankton to detritus absorption coefficients to classify water types, leading to strong correlations between POC and specific optical measurements in certain water samples.
- This method outperforms traditional algorithms and introduces a depth-resolved index for better representation of POC distribution, enhancing our understanding of its three-dimensional structure in ocean environments.
Responses of diatoms and aquatic macroinvertebrates to deposited fine sediment in mesocosm channels as a basis for a new water quality guideline.
Sci Total Environ
December 2024
South Australian Environment Protection Authority, Adelaide 5000, SA, Australia.
Water quality guidelines are an important tool for managing environmental pressures on freshwater streams. Currently, there is a lack of guideline values for fine sediment deposition in South Australian and, more broadly, Australian and overseas streams despite the potentially profound impacts of sediment deposition on aquatic communities. We used an outdoor recirculating stream mesocosm to assess the responses of freshwater diatoms and macroinvertebrates to fine sediment burial after six weeks following a pulsed event.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!