Skin biopsies and blood samples from 117 calves, the offspring of dams that had been pastured on communal Alpine pastures while pregnant, were examined for bovine viral diarrhoea virus (BVDV) antigen. Immunohistochemical evaluation of skin biopsy samples revealed BVDV antigen in nine (7.7%) calves, and ELISA testing of serum samples was positive for BVDV antigen in six (5.1%). Three calves with positive skin biopsy samples and negative serum results were < 11 days old; it was assumed that maternal antibody interfered with the ELISA testing. Serum samples that were collected at a later date from two of the three calves were positive for BVDV antigen. These results were significantly different from those of a previous study in which the prevalence of persistently infected calves in an average Swiss cattle population was 0.64%. It was concluded that the risk of infection with BVDV is high in cattle sharing a communal Alpine pasture.
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http://dx.doi.org/10.1016/j.tvjl.2005.07.018 | DOI Listing |
J Vet Diagn Invest
January 2025
Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA, USA.
Detecting calves that are persistently infected with bovine viral diarrhea virus (BVDV) is essential to disease prevention. Immunohistochemistry (IHC) performed on formalin-fixed, paraffin-embedded ear-notch samples is commonly used for surveillance detection of BVDV antigens. However, due to the low percentage of positive samples in most submissions, the current workflow often entails considerable time reviewing negative results.
View Article and Find Full Text PDFVet J
December 2024
Department of Veterinary Medicine, College of Animal Science, Guizhou University, Guiyang, China. Electronic address:
Bovine viral diarrhea virus (BVDV) is a significant pathogen that exerts substantial economic influence on the global cattle industry. Developing a safe and effective novel vaccine targeting various BVDV subtypes is critical for controlling BVDV infection. In the study, we created two distinct multi-epitope vaccines by linking highly conserved and dominant cytotoxic T-lymphocytes (CTL), helper T-lymphocytes (HTL), and B-cell epitopes from either the E0 or E2 envelope glycoprotein of diverse BVDV subtypes.
View Article and Find Full Text PDFOnderstepoort J Vet Res
December 2024
Department of Biology, College of Science and Arts, Northern Border University, Arar, Saudi Arabia; and, Virology Department, Central Veterinary Research Laboratory, Khartoum.
To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.
View Article and Find Full Text PDFVet Microbiol
January 2025
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China; Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affairs, Daqing 163319, China; Engineering Research Center of Prevention and Control of Cattle Diseases, Daqing, Heilongjiang Province 163319, China. Electronic address:
mBio
December 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
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