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Surgical Transplantation of Tumor Cells into the Spinal Cord of Mice.
J Vis Exp
December 2024
Beijing Institute of Brain Disorders, Capital Medical University; Laboratory of Brain Disorders, Ministry of Science and Technology, Capital Medical University; Collaborative Innovation Center for Brain Disorders, Capital Medical University;
Spinal cord gliomas are commonly malignant tumors of the spinal cord, leading to a high rate of disability. However, uniform treatment guidelines and comprehensive data on spinal cord gliomas remain limited due to the lack of suitable preclinical animal models. Developing a simple and reproducible animal model has become essential for advancing basic and translational research.
View Article and Find Full Text PDFPrediction of axillary lymph node metastasis in breast cancer using an ultrasonic feature- and clinical data-based model.
Am J Cancer Res
December 2024
Department of General Surgery, Liaoning University of Traditional Chinese Medicine Affiliated Hospital Shenyang 110032, Liaoning, China.
The involvement of axillary lymph nodes (ALNs) is a critical prognostic factor affecting patient management and outcomes in breast cancer (BC). This study aims to comprehensively analyze the clinical data of BC patients, evaluate ultrasonic signs of ALNs, and explore the implications of a prediction model for ALN metastasis (ALNM) in early-stage BC patients based on ultrasonic features and clinical data. This study retrospectively analyzed ultrasonic features and clinical data from 216 patients diagnosed with unilateral invasive BC.
View Article and Find Full Text PDFA Reproducible Method for Donor Site Computed Tomography Measurements in Abdominally Based Autologous Breast Reconstruction.
Plast Reconstr Surg Glob Open
January 2025
From the Division of Plastic and Reconstructive Surgery, Washington University School of Medicine in Saint Louis, St. Louis, MO.
We present an approach for evaluating abdominal computed tomography (CT) scans that generates reproducible measures relevant to donor site morbidity after abdominally based breast reconstruction. Seventeen preoperative CT metrics were measured in 20 patients with software: interanterior superior iliac spine distance; abdominal wall protrusion; interrectus distance; rectus abdominis width, thickness, and width-to-thickness ratio; abdominal wall thickness; subcutaneous fat volume; visceral fat volume; right/left psoas volumes and densities; and right/left rectus abdominis volumes and densities. Two operators performed measures to determine interrater reliability (n = 10).
View Article and Find Full Text PDFPsychometric properties of the Short-Form McGill Pain Questionnaire (SF-MPQ) in adult Mexican cancer patients with chronic pain.
Palliat Support Care
January 2025
Department of Psycho-Oncology Service, Instituto Nacional de Cancerología, INCan, Mexico City, Mexico.
Background: Pain is a frequent symptom in cancer patients (CP), and its multidimensional assessment is essential for a comprehensive approach and to establish clinical prognoses. The Short-Form McGill Pain Questionnaire (SF-MPQ) is an internationally recognized tool for the multidimensional assessment of pain, both in clinical and research settings. However, no studies have been reported in Latin America that determine its psychometric properties in CP and chronic pain.
View Article and Find Full Text PDFDeep imaging of LepR stromal cells in optically cleared murine bone hemisections.
Bone Res
January 2025
National Institute of Biological Sciences, Beijing (NIBS), 102206, Beijing, China.
Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional (3D) architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions. Deciphering the spatial interaction of leptin receptor-expressing (LepR) stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue. In this study, we introduce an optimized protocol for the 3D analysis of skeletal tissues, enabling the visualization of hematopoietic and stromal cells, especially LepR stromal cells, within optically cleared bone hemisections.
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