Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR.

Appl Environ Microbiol

Research Department, Aventis Pasteur SA, av. Marcel Mérieux, Marcy L'Etoile 69280, France.

Published: September 2005

Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214643PMC
http://dx.doi.org/10.1128/AEM.71.9.5604-5606.2005DOI Listing

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