AI Article Synopsis
- The research explored how cold stress and immunization affect cytokine gene expression in poultry, focusing on two chicken lines selected for antibody response.
- Cold stress was found to increase the mRNA levels of pro-inflammatory and Th(2) cytokine genes in both chicken lines.
- Immunization using complete Freund's adjuvant led to higher pro-inflammatory cytokine expression, while decreasing Th(1) and Th(2) cytokine levels.
Article Abstract
The effects of cold stress, immunization and genetic selection on the expression of mRNA for cytokine genes in poultry have not been completely elucidated. Therefore, in the present experiment, using real-time quantitative RT-PCR, we evaluated the effect of cold stress and immunization with complete Freund's adjuvant (CFA) on expression of mRNA for pro-inflammatory (interleukin-1beta [IL-1beta], IL-6, IL-12beta), Th(1) (IFN-gamma and IL-2), and Th(2) (IL-4 and IL-10) cytokine genes in peripheral blood leukocytes (PBL) of chicken lines divergently selected for either high or low antibody responses. Irrespective of the duration, cold stress enhanced expression of mRNA for IL-1beta, IL-6, IL-12beta and IL-4 cytokine genes in both selection lines. These results indicate that cold stress stimulates both the innate and parts of the adaptive cellular immune system. Immunization with CFA resulted in higher expression of mRNA for pro-inflammatory cytokines and lower expression of mRNA for both Th(1) and Th(2) cytokines.
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| http://dx.doi.org/10.1016/j.dci.2005.07.001 | DOI Listing |
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