Ultraviolet A exposure alters adhesive properties of mouse melanoma cells.

Background: We have examined whether ultraviolet A (UVA) irradiation could alter adhesive properties of melanoma cells. As an experimental in vitro model, we have used C57BL/6 mouse-derived B16- F1 and B16-F10 melanoma cell lines and the syngeneic MS-1 endothelial cell line.

Method/result: The melanoma cells were exposed to different doses of UVA irradiation. We have determined that a single dose of UVA at 8 and 12 J/cm(2) causes an 88% (P<0.001) and a 32% (P<0.05) increase in B16-F1 melanoma cell adhesiveness to the non-irradiated endothelial monolayer, respectively. The peak of the response was 24 h after the irradiation. The UVA dose of 8 J/cm(2) delivered in four doses separated by 1 h intervals (4 x 2 J/cm(2)) had led to a caused 149% (P<0.001) increase of B16-F1 melanoma adhesiveness already at 1 h after the last dose of UVA. Besides the induction of increase in the melanoma-endothelial cell adhesion, UVA exposure has induced a rapid decline (1 h after exposure) in homotypic melanoma-melanoma cell adhesion (clustering). The clustering decline of B16-F1 cells with a single dose of UVA at 8 J/cm(2) was by 61% (P<0.05) and by 35% (P<0.05) with 4 x 2 J/cm(2). Pilot experiments have shown that the changes of the adhesive properties of melanoma cells were accompanied by an increase in N-cadherin expression and a decline in E-cadherin expression. Such a change in cadherin expression profile has been shown to be an indicator of the increased metastatic potential.

Conclusion: Our results suggest that UVA radiation appears to alter the adhesive properties of melanoma cells in vitro, by diminishing the melanoma-melanoma adhesion and by increasing melanoma adhesion to the endothelium. This suggests that UVA exposure might increase the metastatic capability of the melanoma cells.