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http://dx.doi.org/10.1093/nar/20.11.2897 | DOI Listing |
New Class II restrictases were searched in over 800 microorganisms by using a highly sensitive toluene microtechnique developed in the laboratory. This enabled site-specific endonuclease activity to be revealed in 72 strains. Thirty two new restriction endonucleases were identified, which were highly purified and contained no impurities of nonspecific nucleases, phosphatases.
View Article and Find Full Text PDFNucleic Acids Res
June 1992
Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
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