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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC336949PMC
http://dx.doi.org/10.1093/nar/20.11.2897DOI Listing

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New Class II restrictases were searched in over 800 microorganisms by using a highly sensitive toluene microtechnique developed in the laboratory. This enabled site-specific endonuclease activity to be revealed in 72 strains. Thirty two new restriction endonucleases were identified, which were highly purified and contained no impurities of nonspecific nucleases, phosphatases.

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