A Pto-like gene (designated as SsPto-2) was isolated from Solanum surattense by using genomic walker technology which encoded a cytoplasmically localized serine-threonine protein kinase. Analysis of the 2365 bp segment revealed a gene including a 905 bp 5' flanking region, a 924 bp open reading frame (ORF) and a 536 bp 3' flanking region. The deduced amino acid sequence of the SsPto-2 gene shared high homology with other known Ptos. The deduced SsPto-2 protein contained no signal peptide with a calculated molecular weight of 34.61 kDa. The analysis of SsPto-2 promoter region and terminator region was also presented. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that SsPto-2 transcripts were up-regulated by defense-related factors such as gibberellic acid (GA(3)), salicylic acid (SA) and down-regulated by darkness. The cloning of the SsPto-2 gene will allow us to further study its potential role in disease resistance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10425170500158115 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome-Wide Analysis and Evolution of the Pto-Like Protein Kinase (PLPK) Gene Family in Pepper.
PLoS One
July 2017
Department of Plant Science and Plant Genomics and Breeding Institute, Vegetable Breeding Research Center, Seoul National University, Seoul, 151-921, Korea.
The tomato Pto gene, which encodes a serine/threonine kinase (STK) domain-containing protein, confers resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato (Pst). In this study, in vivo recognition assays using PVX constructs showed that AvrPto was specifically recognized in the pepper genotypes.
View Article and Find Full Text PDFKinase domain-targeted isolation of defense-related receptor-like kinases (RLK/Pelle) in Platanus×acerifolia: phylogenetic and structural analysis.
BMC Res Notes
December 2014
Plant Pathology Research Center, CRA-PAV Agricultural Research Council, V, C,G, Bertero 22, 00156 Rome, Italy.
Background: Plant receptor-like kinase (RLK/Pelle) family regulates growth and developmental processes and interaction with pathogens and symbionts.Platanaceae is one of the earliest branches of Eudicots temporally located before the split which gave rise to Rosids and Asterids. Thus investigations into the RLK family in Platanus can provide information on the evolution of this gene family in the land plants.
View Article and Find Full Text PDFA new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction.
Funct Integr Genomics
August 2013
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Article Synopsis
- New races of coffee rust are challenging existing resistance genes and causing economic difficulties for coffee-producing countries.
- The study aimed to identify genes associated with resistance to coffee rust race II by analyzing a population with a single resistant gene.
- Researchers identified 108 differentially expressed genes related to defense and signaling, with notable increases in gene expression at specific time points post-inoculation, highlighting the role of certain genes like NBS-LRR and thraumatin in resistance.
Silencing of host basal defense response-related gene expression increases susceptibility of Nicotiana benthamiana to Clavibacter michiganensis subsp. michiganensis.
Phytopathology
March 2011
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, NY, USA.
Clavibacter michiganensis subsp. michiganensis is an actinomycete, causing bacterial wilt and canker disease of tomato (Solanum lycopersicum). We used virus-induced gene silencing (VIGS) to identify genes playing a role in host basal defense response to C.
View Article and Find Full Text PDFRegulation of tomato Prf by Pto-like protein kinases.
Mol Plant Microbe Interact
April 2009
The Sainsbury Laboratory, Colney, Norwich, UK.
Tomato Prf encodes a nucleotide-binding domain shared by Apaf-1, certain R proteins, and CED-4 fused to C-terminal leucine-rich repeats (NBARC-LRR) protein that is required for bacterial immunity to Pseudomonas syringae and sensitivity to the organophosphate fenthion. The signaling pathways involve two highly related protein kinases. Pto kinase mediates direct recognition of the bacterial effector proteins AvrPto or AvrPtoB.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!