Objective: To observe the effects of bone marrow endothelial cells on the expansion of human umbilical cord blood (UCB) hematopoietic progenitors in vitro.
Methods: Bone marrow endothelial cells were cultured and the serum free conditioned media (ECM) were collected. CD34+ cells derived from human umbilical cord blood (UCB) were isolated by MiniMACS. Hematopoietic progenitor cells were assayed after culturing with ECM or co-culturing with endothelial cell layer (ECL) for 24 hours. The effects of conditioned medium of endothelial cells which were cultured with bFGF (bFGF-ECM) on the expansion of cord blood hematopoietic progenitors also were investigated.
Results: UCB hematopoietic progenitors were significantly expanded by adding ECM or co-cultured with ECL, and ECM cultures and ECL cultures showed similar expansion effect on early progenitor cells. The effect of bFGF-ECM on the expansion of UCB hematopoietic progenitors was significantly better than that of ECM.
Conclusion: Murine bone marrow endothelial cells support the quantitative expansion of UCB hematopoietic progenitor cells in vitro.
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