AI Article Synopsis
- PMR1 is a Ca2+/Mn2+ ATPase in yeast that is essential for the secretory pathway and is connected to Hailey-Hailey disease due to its human counterpart, hSPCA1, being defective.
- Recent studies highlight pmr1Delta yeast as a valuable model for understanding the molecular pathology of Hailey-Hailey disease, particularly its high calcium sensitivity.
- The study reveals that pmr1Delta yeast has a reduced calcium response to external calcium than wild type strains and shows a novel magnesium sensitivity, suggesting that calcium and magnesium compete for uptake and affect calcium balance in the yeast.
Article Abstract
PMR1, the Ca2+/Mn2+ ATPase of the secretory pathway in Saccharomyces cerevisiae was the first member of the secretory pathway Ca2+ ATPases (SPCA) to be characterized. In the past few years, pmr1Delta yeast have received more attention due to the recognition that the human homologue of this protein, hSPCA1 is defective in chronic benign pemphigus or Hailey-Hailey disease (HHD). Recent publications have described pmr1Delta S. cerevisiae as a useful model organism for studying the molecular pathology of HHD. Some observations indicated that the high Ca2+ sensitive phenotype of PMR1 defective yeast strains may be the most relevant in this respect. Here we show that the total cellular calcium response of a pmr1Delta S. cerevisiae upon extracellular Ca2+ challenge is decreased compared to the wild type strain similarly as observed in keratinocytes. Additionally, the novel magnesium sensitivity of PMR1 defective yeast is revealed, which appears to be a result of competition for uptake between Ca2+ and Mg2+ at the plasma membrane level. Our findings indicate that extracellular Ca2+ and Mg2+ competitively influence the intracellular Ca2+ homeostasis of S. cerevisiae. These observations may further our understanding of HHD.
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| http://dx.doi.org/10.1016/j.femsle.2005.08.017 | DOI Listing |
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