[Effect of HBsAg pulsed dendritic cells on the functions of cytokine-induced killer cells].

Aim: To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells.

Methods: The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells.

Results: The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells (P<0.05).

Conclusion: HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.