Objective: To study the significance of the change of protamine content in the management of male infertility.
Methods: Sperm nuclear proteins extracted from 199 infertile patients were analyzed by polyacrylamide gel-electrophoresis and scanning microdensitometry.
Results: Forty-two (21%) cases of the total number had normal sperm nuclear proteins and 157 (79%) had aberrant ones, which mainly presented the interruption of HPRR and abnormality of P2 protamine. Thirty patients were selected at random from 157 abnormal cases for clinical treatment. After the treatment, the sperm nuclear proteins were extracted and analyzed and the results demonstrated that 11 cases (36.6%) improved markedly, 5 (16.6%) restored to normal and the other 16 (46.6%) remained unchanged.
Conclusion: There is a reduced level or selective absence or even complete selective absence of protamines in infertile patients. Protamines may act as a parameter for evaluating the treatment effect of infertile males and protamine content can be influenced by a certain or several factors.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Histone variants: The bricks that fit differently.
J Biol Chem
December 2024
Department of Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, USA. Electronic address:
Histone proteins organize nuclear DNA in eukaryotic cells and play crucial roles in regulating chromatin structure and function. Histone variants are produced by distinct histone genes and are produced independently of their canonical counterparts throughout the cell cycle. Even though histone variants may differ by only one or a few amino acids relative to their canonical counterparts, these minor variations can profoundly alter chromatin structure, accessibility, dynamics, and gene expression.
View Article and Find Full Text PDFSemen cryopreservation and storage in liquid nitrogen: Impact on chromatin compaction.
Andrology
November 2024
Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Florence, Italy.
Article Synopsis
- Sperm cryopreservation serves as an option for preserving male fertility, but the freezing and thawing processes can harm sperm viability, motility, and DNA integrity, with less focus on chromatin structure.
- This study aimed to assess how cryopreservation affects sperm chromatin compaction and other sperm parameters in relation to storage time in liquid nitrogen.
- Findings revealed that after 7 days of storage, there was a notable decrease in sperm motility and viability, and an increase in chromatin compaction and DNA fragmentation, indicating that while motility declines, chromatin structure changes can be significant.
N-acetyl-L-cysteine reduces testis ROS in obese fathers but fails in protecting offspring from acquisition of epigenetic traits at and ICR loci.
Front Cell Dev Biol
October 2024
Department of Pharmacy, University of Naples "Federico II", Naples, Italy.
Introduction: Paternal nutrition before conception has a marked impact on offspring's risk of developing metabolic disorders during adulthood. Research on human cohorts and animal models has shown that paternal obesity alters sperm epigenetics (DNA methylation, protamine-to-histone replacement, and non-coding RNA content), leading to adverse health outcomes in the offspring. So far, the mechanistic events that translate paternal nutrition into sperm epigenetic changes remain unclear.
View Article and Find Full Text PDF"Rich arginine and strong positive charge" antimicrobial protein protamine: From its action on cell membranes to inhibition of bacterial vital functions.
Biochim Biophys Acta Biomembr
June 2024
Graduate School of Science and Technology, Meiji University, 1-1-1, Higashimita, Tama-ku, Kawasaki 214-8571, Kanagawa, Japan. Electronic address:
Protamine, an antimicrobial protein derived from salmon sperm with a molecular weight of approximately 5 kDa, is composed of 60-70 % arginine and is a highly charged protein. Here, we investigated the mechanism of antimicrobial action of protamine against Cutibacterium acnes (C. acnes) focusing on its rich arginine content and strong positive charge.
View Article and Find Full Text PDFEffect of Advanced Glycation end Products (AGEs) on Sperm Parameters and Function in C57Bl/6 Mice.
Reprod Sci
July 2024
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
This study investigated the deleterious impact of advanced glycation end products (AGEs), commonly present in metabolic disorders like diabetes, obesity, and infertility-related conditions, on sperm structure and function using a mouse model where AGE generation was heightened through dietary intervention. Five-week-old C57BL/6 mice were divided into two groups, one on a regular diet (control) and the other on an AGE-rich diet. After 13 weeks, various parameters were examined, including fasting blood glucose, body weight, food consumption, sperm parameters and function, testicular superoxide dismutase levels, malondialdehyde content, total antioxidant capacity, Johnson score, AGE receptor (RAGE) content, and carboxymethyl lysine (CML) content.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!