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Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454). | LitMetric
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Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).

Jun-ichiro Sekiguchi Prasit Tharavichitkul Tohru Miyoshi-Akiyama Vena Chupia Tomoko Fujino Minako Araake Atsushi Irie Koji Morita Tadatoshi Kuratsuji Teruo Kirikae

Antimicrob Agents Chemother

Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.

Published: September 2005

A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1195413PMC
http://dx.doi.org/10.1128/AAC.49.9.3948-3951.2005DOI Listing

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