DNA fingerprinting of Wistar rat were studied with JL-02 Mulilocus probe and Southern hybridization, and comparing with different individuals of nine groups Wistar rat from six national classical area and different groups. It was indicated that DNA fingerprinting could reflect genetic material of outbred strain rat,and were more polymorphic. The genetic distances of Wistar rat were distributed for 0.2-0.6 among different individuals within same groups, and the genetic distances of Wistar rat were distributed for 0.2-0.7 among different groups.

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