A novel method for preparation of DNA tubes using the DNA tile system with assistance of a four-way branched DNA-porphyrin connector is described. The detailed DNA tube structures were characterized by atomic force microscopy (AFM).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477613 | PMC |
| http://dx.doi.org/10.1002/anie.200501034 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
DNA Tetrahedron-enhanced single-particle counting integrated with cascaded CRISPR Program for ultrasensitive dual RNAs logic sensing.
J Colloid Interface Sci
December 2024
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People's Republic of China.
CRISPR-Cas-based technology, emerging as a leading platform for molecular assays, has been extensively researched and applied in bioanalysis. However, achieving simultaneous and highly sensitive detection of multiple nucleic acid targets remains a significant challenge for most current CRISPR-Cas systems. Herein, a CRISPR Cas12a based calibratable single particle counting-mediated biosensor was constructed for dual RNAs logic and ultra-sensitive detection in one tube based on DNA Tetrahedron (DTN)-interface supported fluorescent particle probes coupled with a novel synergistic cascaded strategy between CRISPR Cas13a system and strand displacement amplification (SDA).
View Article and Find Full Text PDFA Multiplex High-Resolution Melting (HRM) assay to differentiate Fusarium graminearum chemotypes.
Sci Rep
December 2024
Cereal Disease Laboratory, Agricultural Research Service, US Department of Agriculture, St. Paul, MN, 55108, USA.
Fusarium graminearum is a primary cause of Fusarium head blight (FHB) on wheat and barley. The fungus produces trichothecene mycotoxins that render grain unsuitable for food, feed, or malt. Isolates of F.
View Article and Find Full Text PDFInert splint-driven oligonucleotide assembly.
Synth Biol (Oxf)
December 2024
Claret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA 95066, United States.
In this study, we introduce a new method for oligonucleotide fragment assembly. Unlike polymerase chain assembly and ligase chain assembly that rely on short, highly purified oligonucleotides, our method, named , uses a one-tube, splint-driven assembly reaction. Splynthesis connects standard-desalted "contig" oligos (∼150 nt in length) via shorter "splint" oligos harboring 5' and 3' blocking modifications to prevent off-target ligation and amplification events.
View Article and Find Full Text PDFAbout bacteriophage tail terminator and tail completion proteins: structure of the proximal extremity of siphophage T5 tail.
J Virol
December 2024
Université Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
Bacteriophages are viruses infecting bacteria. The vast majority of them bear a tail, allowing host recognition, cell wall perforation, and DNA injection into the host cytoplasm. Using electron cryo-microscopy (cryo-EM) and single particle analysis, we determined the organization of the tail proximal extremity of siphophage T5 that possesses a long flexible tail and solved the structure of its tail terminator protein p142 (TrP).
View Article and Find Full Text PDFRemodeling of Individual Nucleosomes in Nucleosome Arrays.
Methods Mol Biol
December 2024
Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Adenosin triphosphate (ATP)-dependent nucleosome remodeling factors sculpt the nucleosomal landscape of eukaryotic chromatin. They deposit, evict, or reposition nucleosomes along DNA in a process termed nucleosome sliding. Remodeling has traditionally been analyzed using mononucleosomes as a model substrate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!