AI Article Synopsis
- The study examined the relationship between the optical quality of bovine lenses and mitochondrial health using a chemical called CCCP, which disrupts mitochondria.
- Exposure to CCCP led to a dose- and time-dependent decrease in the lenses' ability to focus sharply.
- Lenses exposed to lower concentrations of CCCP showed signs of recovery after 24 hours, while the highest concentration resulted in noticeably damaged mitochondria in specific lens cells, highlighting a strong link between lens optical function and mitochondrial integrity.
Article Abstract
In order to elucidate the correlation between lens optical function and metabolic function, in vitro bovine lens optical quality and mitochondrial integrity was measured following treatment with carbonyl cyanide m-chlorophenylhydrazone (the mitochondrial depolarizing agent, CCCP). The results indicate that in vitro exposure to CCCP resulted in concentration and time-dependent loss of sharp focus. The concentrations tested included 65.0, 32.5, 16.25 and 8.125 microm CCCP. Lenses treated with two lower concentrations show recovery from damage at the 24-h scan point. In lenses treated with 65 microM CCCP, mitochondria in lens epithelial and superficial cortical fibre cells appeared short and swollen. The results of this study indicate that lens optical function and mitochondrial integrity are closely correlated.
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| http://dx.doi.org/10.1016/S1567-7249(03)00059-X | DOI Listing |
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