AI Article Synopsis
- Mitochondria in osteosarcoma cells (143B) show different shapes and sizes, categorized as reticular, fragmented, or intermediate, and their morphology changes throughout the cell cycle.
- In G1 phase, mitochondria are reticular, while in S phase, they become fragmented, indicating a dynamic relationship tied to the cell cycle.
- A new fluorescence in situ hybridization technique highlighted that mitochondrial DNA (mtDNA) clusters are linked to these morphologies, emphasizing the role of mitochondrial fission and fusion in maintaining their structure and function.
Article Abstract
Mitochondria of osteosarcoma cells (143B) in culture have variable morphologies, classified according to the shape and size of the organelle as reticular, fragmented or intermediate. Synchronization and release from G0 has shown that the morphology of mitochondria oscillates between the reticular and fragmented state in a cell cycle dependent manner. Cells in G1 have reticular mitochondria while those in S phase have fragmented mitochondria. By using a novel method of fluorescence in situ hybridization, the morphology of mitochondria was correlated with mitochondrial DNA distribution. MtDNA molecules were seen in clusters of two to four along mitochondrial filaments. In the fully fragmented state, each mitochondrion contained at least one cluster. We discuss the importance of fission and fusion events in regulating the morphology of mitochondria, segregation of mtDNA and maintenance of the organelle's functional unity.
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| http://dx.doi.org/10.1016/s1567-7249(02)00006-5 | DOI Listing |
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